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Description:Brains were cut into 1-mm3 pieces with a razor blade and pieces further ground to dust with a pestle and mortar. Immunofluorescence staining was performed as previously described Nakaya et al. The proteolytic activity of presenilins cleaves Type-I transmembrane proteins at sites within their respective membrane-spanning domains 1 , 6 , 7. Both software packages also contain a feature that was used to correct raw iTRAQ ratios for impurity levels of individual iTRAQ reagent lots determined by the manufacturer.